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The relative luciferase activity of three regions of PRMT1 gene promoter in BEAS 2B or HFL 1 cells were detected by the dual luciferase reporter assay after transfection pPRMT1 for 48h with or without IL 4 or IL 1 incubated for 24h (C,D). The experiment was performed in quintuplicate and values represent the average of three different experiments after normalization against Renilla activity. The data were expressed as mean SEM and analyzed by One way ANOVA test.
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