Method name: praziquantel tablets – praziquantel – High Performance Liquid Chromatography
Applications: The method for determining the content of praziquantel praziquantel tablets using high performance liquid chromatography.
This method is applicable to praziquantel tablets.
Method: the small study sample, internal standard solution dissolved in methanol and then diluted into the HPLC chromatography with UV absorbance detector at a wavelength of 263nm peak area detected praziquantel, calculated its content.
Reagents: 1 methanol
2. Α-asarone
Equipment: 1 Instrument
1.1 HPLC
1.2 column
Octadecylsilane bonded silica as a filler
1.3 UV absorption detector
2 chromatographic conditions
2.1 mobile phase: methanol-water = 10040
2.2 detection wavelength: 263nm
2.3 column temperature: room temperature
Preparation of internal standard solution 1: Sample Preparation.
Precision Weigh α-asarone amount, dissolved in methanol and diluted into each 1mL solution containing 0.4mg, namely the internal standard solution.
Preparation of 2 reference solution
Precision Weigh praziquantel reference about 100mg, set 10mL volumetric flask, precision internal standard solution 5mL, and shake to dissolve, diluted with methanol to the mark, shake, is the reference solution.
Preparation of the test solution 3.
20 to take the test, accurately weighed, small study, accurately weighed amount (approximately equivalent to praziquantel 100mg), set 10mL volumetric flask, precision internal standard solution 5mL, and shake to dissolve, dilute with methanol to scale, shake, that is, the test solution.
Note: “Precision Weigh” means to check the weight should be accurately referred to take the weight of one-thousandth. ‘Precise amount “means the amount of the volume should meet the accuracy requirements of national standards for the volume pipettes.
Steps: precision drawing reference solution and the test solution 10mL, high performance liquid chromatography with UV absorbance detector praziquantel (C19H24N2O2) at a wavelength of 263nm peak area, calculates its content.
